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KMID : 0880220110490030499
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Journal of Microbiology
2011 Volume.49 No. 3 p.499 ~ p.501
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A modified immunoblot method to identify substrates of protein kinases
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Kang Choong-Min
Wan Jin Jahng
Robert N. Husson
Lee Sang-Hee
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Abstract
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While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis.
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KEYWORD
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modified immunoblot method, substrates of kinases, partial transfer of proteins
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